Lck PE MOL 171 50Tst

• 产品编号：BD-558496      品牌：BD-Pharmingen       原厂货号：558496
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Lck is a member of the Src family of cytoplasmic protein-tyrosine kinases (PTKs)
that is normally expressed exclusively in lymphoid cells, primarily T lymphocytes
and NK cells. A low level of expression has been detected in B lymphocytes, but its
function in B cells is unknown. Its expression in other leukocytes is not well
defined. Members of the Src family have several common features: 1) unique
N-terminal domains, 2) attachment to cellular membranes through a myristylated
N-terminus, and 3) homologous SH2, SH3, and catalytic domains. The unique
N-terminal domain of Lck interacts with the cytoplasmic tails of the CD4 and CD8
cell-surface glycoproteins of T lymphocytes, which recognize antigen presenting cells
via their surface MHC class II and class I molecules, respectively. The catalytic
activity of Lck is regulated by both kinases and phosphatases that control the
phosphorylation states of two tyrosine residues that have opposing effects.
Repression of Lck's catalytic activity occurs via phosphorylation at tyrosine 505
(Y505), located near the carboxy terminus. Phosphorylation of this tyrosine site is
mediated by the Csk family of PTKs, and its dephosphorylation is mediated by the
protein tyrosine phosphatase, CD45. When Lck is phosphorylated at this site, it
assumes a folded tertiary structure which is enzymatically inactive. When CD45
dephosphorylates it at Y505, Lck is able to autophosphorylate its Y394, which leads
to conformational changes in the catalytic domain that induce kinase activity.
However, it has been observed that the inhibitory effect of the phosphorylated Y505
can be overcome by direct engagement of Lck's SH3 domain and that both Y394 and
Y505 are phosphorylated together in cells activated by hydrogen peroxide. Activated
Lck phosphorylates the ITAMs (Immunoreceptor-based Tyrosine Activation Motifs)
of the T cell receptor (TCR) and thus is critical for activation and development of T
lymphocytes. The interactions of Lck, Csk, CD45, CD4 or CD8, and TCR are only a
small part of a complex immunoregulatory cascade that involves additional
substrates for Csk and CD45, other enzymes, adhesion molecules, adaptor proteins,
and specialized membrane microdomains.
The MOL 171 monoclonal antibody recognizes the 56- and 60-kDa forms of human
Lck protein, regardless of phosphorylation status. It cross reacts with mouse Lck.

原厂资料：

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
5.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.