The BD IMag™ Mouse CD8 T Lymphocyte Enrichment Set - DM is used for the negative selection of CD8 T lymphocytes from mouse spleen or lymph node. The Biotinylated Mouse CD8 T Lymphocyte Enrichment Cocktail contains monoclonal antibodies that recognize antigens expressed on peripheral erythrocytes and leukocytes that are not CD8 T lymphocytes. The BD IMag Streptavidin Particles Plus - DM are magnetic nanoparticles that have streptavidin covalently conjugated to their surfaces. With these two components, the BD IMag Mouse CD8 T Lymphocyte Enrichment Set -DM avoids the inadvertent activation of the enriched CD8 T lymphocytes by using reagents that do not directly bind to those CD8 T cells. It has not been tested on the activated mouse cells. This Set has been optimized for use with the BD IMagnet, and it contains sufficient reagents to label 10^9 leukocytes. The CD8 T Lymphocyte Enrichment Cocktail component is comprised of the following biotin-conjugated monoclonal antibodies: Anti-mouse CD4, clone GK1.5 Anti-mouse CD11b, clone M1/70 Anti-mouse CD45R/B220, clone RA3-6B2 Anti-mouse CD49b, clone HMα2 Anti-mouse TER-119/Erythroid Cells, clone TER-119
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注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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