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Hu Monocyte Enrichment Set IMag- DM

 
包装: 5mL
运保温度: Store undiluted at 4°C. Antibody or streptavidin w
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其它组分:

Human Monocyte Enrichment Cocktail【子货号:51-9004592】
Biotin Mouse Anti-Human CD41a【子货号:51-9004594】
Streptavidin Particles Plus - DM【子货号:51-9000810】


描述:

The BD IMag™ Human Monocyte Enrichment Set - DM is used for the negative selection of monocytes from peripheral blood. The Human Monocyte Enrichment Cocktail (component 51-9004592) contains biotinylated monoclonal antibodies that recognize antigens expressed on erythrocytes, and peripheral leukocytes. The Human Monocyte Enrichment Cocktail (component 51-9004592) consists of the following biotin-conjugated anti-human antibodies: CD3 (clone UCHT1), CD45RA (clone HI100), CD19 (clone HIB19), CD56 (clone B159), and CD235a (GA-R2). An additional reagent is also included (component 51-9004594), Biotin Mouse anti-human CD41a, offering investigators an option of removing platelets. Platelets tend to stick to monocytes and therefore the addition of CD41a may lower the yield of monocytes after the enrichment procedure, but the resulting monocyte population will be more pure than without the addition of the anti-human CD41a antibody. The BD IMag™ Streptavidin Particles Plus - DM (component 51-9000810) are magnetic nanoparticles that have streptavidin covalently conjugated to their surfaces. The BD IMag™ Human Monocyte Enrichment Set - DM has been optimized for use with the BD IMagnet™, and it contains sufficient reagents to label 10e9 peripheral blood mononuclear cells (PBMC).


注意事项:

Store undiluted at 4°C. Antibody or streptavidin was conjugated to the magnetic particles under optimum conditions, and unconjugated antibody/streptavidin was removed.


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