The BD IMag™ Mouse Hematopoietic Progenitor Cell Enrichment Set - DM reacts with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, and erythrocytes. The Biotinylated Mouse Lineage Depletion Cocktail contains biotinylated monoclonal antibodies to mouse CD3e (CD3 ε chain), CD11b (Integrin αM chain), CD45R/B220, Ly-6G and Ly-6C (Gr-1), and TER-119/Erythroid Cells (Ly-76). The BD IMag™ Streptavidin Particles Plus - DM are magnetic nanoparticles that have streptavidin covalently conjugated to their surfaces. This Set is designed for the immunomagnetic enrichment of hematopoietic progenitors from mouse bone marrow by depletion of cells committed to the T- and B-lymphocytic, myeloid (monocytic and granulocytic), and erythroid lineages. The Set contains sufficient reagents to label 10^9 bone marrow cells. Biotin Mouse Lineage Depletion Cocktail is comprised of the following biotin-conjugated monoclonal antibodies: Anti-mouse CD3e, clone 145-2C11 Anti-mouse CD11, clone M1/70 Anti-mouse CD45R/B220, clone RA3-6B2 Anti-mouse Ly-6G and Ly-6C (Gr-1), clone RB6-8C5 Anti-mouse TER-119/Erythroid Cells, clone TER-119
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Antibody or streptavidin was conjugated to the magnetic particles under optimum conditions, and unconjugated antibody/streptavidin was removed. Store undiluted at 4°C