Ms/Rat TNF Biotin 516D1A1 500ug

产品编号：BD-558415 品牌：BD-Pharmingen 原厂货号：558415
产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
应用分类：

包装：

500ug

运保温度:

Store undiluted at 4°C

到货周期：

登录后查看

标准价：

￥客户可见

会员价：

￥客户可见

积分：

客户可见分

登录之后可加入购物车购买，请您

运费与支付说明：

1.含干冰类产品有运费；

2. 必须现金付款或有信用额度的会员才可以直接发货，否则需要等待现金付款信息。

描述：

The 516D1A1 Fab antibody reacts with mouse and rat tumor-necrosis factor (TNF, formerly known as TNF-α) proteins. The 516D1A1 Fab
was selected by screening a human Fab phage display library (Hoet et al.) licensed from Dyax Corp (Cambridge, MA) with recombinant
mouse TNF protein. The biology and function of the TNF protein has been extensively reviewed (Hehlgans et al.) in the literature.
The biotinylated 516D1A1 antibody is useful as a detection antibody for a sandwich ELISA that measures mouse and rat TNF protein levels.
Purified Hamster Anti-Mouse/Rat TNF (Cat. No.557516) as capture antibody can be paired with biotinylated 516D1A1 as the detection
antibody, using purified recombinant mouse TNF protein (Cat. No. 554589) or purified recombinant rat TNF protein (Cat. No. 555109) as
standard. The biotinylated 516D1A1 antibody should be titrated between 0.25 - 2.0 μg/ml to determine its optimal ELISA detection
concentration. To obtain linear standard curves, doubling dilutions of purified mouse or rat TNF protein ranging from 31.25 pg/ml to 2,000
pg/ml are recommended for inclusion in each ELISA plate. Investigators may find methodology information to be useful in Techniques for
Immune Function Analysis Application Handbook 1st Edition. BD Biosciences (2003)-- Chapter 7: ELISA for specifically measuring the
levels of cytokines, chemokines, and inflammatory mediators and their receptors. For this ELISA antibody pair, no cross-reactivity has been
observed with the following: Rat IL-1α, IL-2, IL-4, IL-6, IL-10, IL-18, GM-CSF, IFN-γ; mouse IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6,
IL-7, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17, IL-18, M-CSF, GM-CSF, IFN-γ, MCP-1, MIP-1α, MIP-1β, sTNFRI, sTNFRII, RANTES;
human TNF.

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. .
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.