Ms T Lym Subset Ab Cktl w/ItCl 100Tst

• 产品编号：BD-558391      品牌：BD-Pharmingen       原厂货号：558391
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

描述：

The Mouse T Lymphocyte Subset Antibody Cocktail is a three-color reagent designed to identify major subsets of T lymphocytes by direct
immunofluorescent staining with flow cytometric analysis. This cocktail consists of the following antibody mixture: PE-Cy7 hamster
anti-mouse CD3e (clone 145-2C11), PE rat anti-mouse CD4 (clone RM4-5), and FITC rat anti-mouse CD8a (clone 53-6.7).The 145-2C11
antibody reacts with the 25-kDa echain of the T-cell receptor-associated CD3 complex, which is expressed on thymocytes, mature T
lymphocytes, and NK-T cells of all mouse strains tested. The RM4-5 antibody recognizes CD4 (L3T4), a differentiation antigen expressed on
most thymocytes, subpopulations of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T-helper cells and
immunosuppressive regulatory T cells), and a subset of NK-T cells in all mouse strains tested. CD4 has also been detected on pluripotent
hematopoietic stem cells, bone-marrow myeloid and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic
dendritic cells. The 53-6.7 antibody reacts with the 38-kDa α and 34-kDa α' chains of the CD8 differentiation antigen (Ly-2 or Lyt-2)
expressed on most thymocytes, subpopulations of mature T lymphocytes (including MHC class I-restricted T suppressor/cytotoxic cells and
subsets of γδ TCR-bearing cells and intestinal intraepithelial lymphocytes) of all mouse strains tested. CD8a is also expressed on a subset of
dendritic cells. The three antibodies have been titrated and pre-diluted, mixed together, and formulated for optimal staining performance. The
Mouse T Lymphocyte Subset Isotype Control contains equivalent concentrations of fluorochrome- and isotype-matched negative-control
immunoglobulin.
The use of three different fluorochromes for the labeling of the three different antibodies permits the recognition of each of the three antigens
on each cell in a sample. The levels of expression of the three antigens distinguish the major subpopulations of developing and peripheral T
lymphocytes. Additional fluorochrome-labeled reagents may be combined with the Mouse T Lymphocyte Subset Antibody Cocktail, and the
Mouse T Lymphocyte Subset Isotype Control, to further characterize T-cell subpopulations.
The Mouse T Lymphocyte Subset Isotype Control contains equivalent concentrations of fluorochrome- and isotype-matched negative-control
immunoglobulin consisting of the following: PE-Cy7 Armenian hamster IgG1, κ (clone A19-3), and PE and FITC Rat IgG2a, κ (clone
R35-95).

原厂资料：

注意事项：

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
The antibody was conjugated with PE-Cy7 under optimum conditions, and unconjugated antibody and free PE-Cy7 were removed.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.