Hu Regulatory TLym Separation Set-DM

• 产品编号：BD-558142      品牌：BD-Pharmingen       原厂货号：558142
• 产品分类：生化和细胞分析 > 微珠和磁珠 > 细胞分选磁珠 > 分离人细胞
• 应用分类：

描述：

The BD IMag™ Human Regulatory T Lymphocyte Separation Set – DM is used for the isolation of CD25+ CD4+ regulatory T lymphocytes (Treg cells) from peripheral blood. The Human Regulatory T Lymphocyte Separation Cocktail contains biotinylated monoclonal antibodies (mAb) that recognize antigens expressed on erythrocytes, platelets, and peripheral leukocytes that are not CD4 T lymphocytes. It also contains anti–human CD25 mAb that is labeled with allophycocyanin (APC). After peripheral blood mononuclear cells (PMBC) are labeled with the cocktail, the CD4+ CD25+ Treg cells are isolated in two immunomagnetic separation steps. First, the Streptavidin Particles Plus – DM enrich the CD4+ T lymphocytes by negative selection (depletion of the non-CD4+ cells). In the second immunomagnetic separation step, the Anti-APC Particles – DM select the CD25+ cells, from among the enriched CD4+ cells, which already had been labeled with the APC anti-CD25 mAb. This Separation Set has been optimized for use with the BD IMagnet™, and it contains sufficient reagents to label 109 PBMC.

原厂资料：

注意事项：

The monoclonal antibody cocktail should be protected from prolonged exposure to light and stored undiluted at 4˚C. The streptavidin– and antiAPC–conjugated magnetic particles should be stored undiluted at 4˚C. Do not freeze.