The BD IMag™ Mouse CD4 T Lymphocyte Enrichment Set - DM is used for the negative selection of CD4 T lymphocytes from mouse spleen or lymph node. The Biotinylated Mouse CD4 T Lymphocyte Enrichment Cocktail contains monoclonal antibodies that recognize antigens expressed on peripheral erythrocytes and leukocytes that are not CD4 T lymphocytes. The BD IMag Streptavidin Particles Plus - DM are magnetic nanoparticles that have streptavidin covalently conjugated to their surfaces. With these two components, the BD IMag Mouse CD4 T Lymphocyte Enrichment Set -DM avoids the inadvertent activation of the enriched CD4 T lymphocytes by using reagents that do not directly bind to those CD4 T cells. This Set has been optimized for use with the BD IMagnet, and it contains sufficient reagents to label 10^9 leukocytes. The CD4 T Lymphocyte Enrichment Cocktail set is comprised from the following biotin-conjugated monoclonal antibodies: Anti-mouse CD8a, clone 53-6.7 Anti-mouse CD11b, clone M1/70 Anti-mouse CD45R/B220, clone RA3-6B2 Anti-mouse CD49b, clone HMα2 Anti-mouse TER-119/Erythroid Cells, clone TER-119
原厂资料:
注意事项:
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.