Phosflow Perm Buffer II 125mL

包装：

125mL

运保温度:

Store undiluted at room temperature.

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描述：

BD Phosflow™ Perm Buffer II is intended to be used for the permeabilization of fixed PBMC's or lysed whole blood. PBMC's, for example,

can be stimulated with kinase activators, such as phorbol esters, and can be fixed with BD Phosflow™ Fix Buffer I (Cat. No. 557870)

followed by permeabilization of the cells with BD Phosflow™ Perm Buffer II to allow for an effective intracellular staining of kinases.

Designed for optimal fluorescent staining in flow cytometry, BD Phosflow™ Perm Buffer II can be used with antibodies for phospho-protein

analysis (i.e the staining of intracellular proteins involved in cell signalling pathways, such as phosphorylated proteins and assorted kinases).

In many cases, these phospho-specific antibodies may be used simultaneously with the staining of cell surface markers (e.g CD markers). Not

all cell surface markers, however, are compatible with BD Phosflow™ Perm Buffer II. For a current listing of compatible cell surface markers

for use with BD Phosflow™ Perm Buffer II, investigators are encouraged to visit the following website:

http://www.bdbiosciences.com/documents/antibodies_human_cellsurface_marker.pdf.

注意事项：

Store undiluted at room temperature.