Ms Early B Lineage PE AA4.1 100ug

产品编号：BD-558039 品牌：BD-Pharmingen 原厂货号：558039
产品分类：抗体 > 一抗 > 染料标记抗体
应用分类：

包装：

100ug

运保温度:

Store undiluted at 4°C

到货周期：

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描述：

The AA4.1 antibody reacts with a 130-140-kDa type I transmembrane protein expressed on immature B lymphocytes in the adult bone

marrow; and on hematopoietic progenitors and stem cells in adult bone marrow, fetal liver, and embryonic yolk sac. Although staining of

splenic immature/transitional B cells has been reported, we find that the antigen density is much lower in the spleen than in the bone marrow.

Staining of spleen requires amplification through the use of a second step. The FITC conjugate of mAb AA4.1, while ideal for bone marrow

staining, is not effective in the spleen (please see Usage comments below). It has been observed that the staining pattern of mAb 493 (Cat.

Nos. 550433 and 550434 for the purified and biotinylated formats, respectively) is similar to that of mAb AA4.1, that both antibodies

precipitate molecules of the same molecular weight, and that staining by mAb AA4.1 is not blocked by mAb 493, suggesting that the

antibodies recognize separate epitopes of the same Early B Lineage antigen.

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

4.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

5.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.