Ms IgG1 IMag Particles-DM A85-1 5mL

• 产品编号：BD-557983      品牌：BD-Pharmingen       原厂货号：557983
• 产品分类：生化和细胞分析 > 微珠和磁珠 > 细胞分选磁珠 > 分离鼠细胞
• 应用分类：

描述：

BD IMag™ Anti-Mouse IgG1 Magnetic Particles -DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces.
The A85-1 clone has been reported to react specifically with mouse IgG1 of Igh-Ca and Igh-Cb haplotypes, but reportedly does not react with
other Ig isotypes. These particles are optimized for the positive selection or depletion of leukocyte subpopulations labelled with mouse IgG1
antibodies using the BD IMagnet™.
Leukocytes are labelled with BD IMag™ Anti-Mouse IgG1 Magnetic Particles - DM according to the Magnetic Labeling and Separation
Protocol. In brief, cells are labeled with a mouse IgG1 antibody that recognizes the subpopulation of interest. After washing away excess
antibody, BD IMag™ Anti-Mouse IgG1 Magnetic Particles -DM are added to the cell suspension and bind the mouse IgG1 antibody on the
cells. This labeled cell suspension is then placed within the magnetic field of the BD IMagnet™ (Cat.No. 552311). Positive selection or
depletion is then performed. Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can
be drawn off (depleted or negative fraction). The tube is then removed from the magnetic field for resuspension of the positive fraction. The
magnetic separation steps are diagrammed in the Depletion and Positive Selection Flow Charts. After the positive fraction has been washed,
the small size of the magnetic particles allows the positive fraction to be further evaluated in downstream applications such as flow cytometry.

原厂资料：

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.