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Hu CD45RA IMag Particles-DM HI100 5mL

 
包装: 5mL
运保温度: Store undiluted at 4°C
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描述:

BD™ IMag Anti-Human CD45RA Particles - DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces.
These particles are optimized for the positive selection or depletion of the CD45RA-bearing leukocytes using the BD™ IMagnet (Cat. No.
552311). CD45RA is the 220 kDa isoform of the human leukocyte common antigen found on major subsets of peripheral CD4+ and CD8+ T
lymphocytes, B lymphocytes, NK cells, and monocytes. CD45RO and CD45RA expression defines complementary, predominantly
non-overlapping populations of T cells in peripheral blood; and it is generally accepted that naive T cells are CD45RO- CD45RA+, while
memory T cells are CD45RO+ CD45RA-. To specifically enrich CD45RA-expressing naive T lymphocytes, we recommend first depleting the
erythrocytes, platelets, and non-T leukocytes, by using the appropriate BD™ IMag human T lymphocyte enrichment set, followed by positive
selection of the CD45RA+ population (please refer to the following protocol).


注意事项:


1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.


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