Ms T Lympho Act Cktl w/ItCl 100Tst

产品编号：BD-557916 品牌：BD-Pharmingen 原厂货号：557916
产品分类：抗体 > 一抗 > 复合抗体试剂盒
应用分类：

包装：

100Tst

运保温度:

Store undiluted at 4°C

到货周期：

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描述：

The Mouse T Lymphocyte Activation Antibody Cocktail is a three-color reagent designed to identify major subsets of T lymphocytes by direct
immunofluorscent staining using flow cytometric analysis. This cocktail consists of the following antibody mixture: PE-Cy™7 rat anti-mouse
CD25 (clone PC61), PE Armenian hamster anti-mouse CD69 (clone H1.2F3), and FITC Armenian hamster anti-mouse CD3ε (clone
145-2C11). PE-Cy™7 rat anti-mouse CD25 (clone PC61) reacts with CD25, the low-affinity IL-2 receptor α chain (IL-2Rα, p55) expressed
on activated mouse T and B lymphocytes. CD25 is also found on some developing B cells in the bone marrow, early developing T cells in the
thymus, peripheral CD4+ regulatory T (Treg) cells, and dendritic cells. PE hamster anti-mouse CD69 (clone H1.2F3) reacts with CD69, also
known as the very early activation antigen. Its expression is rapidly induced upon activation of lymphocytes (T, B, NK, and NK-T cells),
neutrophils, and macrophages. CD69 has also been reported to be expressed on thymocytes that are undergoing positive selection. FITC
hamster anti-mouse CD3ε (clone 145-2C11) reacts with the 25 kDa ε chain of the T-cell receptor-associated CD3 complex, expressed on
thymocytes, mature T lymphocytes, and NK-T cells.
The Mouse T Lymphocyte Activation Isotype Control contains equivalent concentrations of fluorochrome- and isotype-matched
negative-control immunoglobulin consisting of the following: PE-Cy™7 rat IgG1, λ (clone A110-1), PE Armenian hamster IgG1, λ (clone
G235-2356) and FITC Armenian hamster IgG1, κ (clone A19-3).

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.