描述：
The immunofluorescent staining of incorporated
bromodeoxyuridine (BrdU) and flow cytometric analysis
provide a high-resolution technique to determine the
frequency and nature of individual cells that have
synthesized DNA. In this method, BrdU (an analog of
the DNA precursor thymidine) is incorporated into
newly synthesized DNA by cells entering and progressing
through the S (DNA synthesis) phase of the cell cycle.1-4
The incorporated BrdU is stained with specific anti-BrdU
fluorescent antibodies. The levels of cell-associated BrdU
are then measured by flow cytometry. Often, staining
with a dye that binds to total DNA such as 7-aminoactinomycin
D (7-AAD) is coupled with
immunofluorescent BrdU staining. With this
combination, two-color flow cytometric analysis permits
the enumeration and characterization of cells that are
actively synthesizing DNA (BrdU incorporation) in terms
of their cell cycle position (ie, G0/1, S, or G2/M phase
defined by 7-AAD staining intensities).5,6
Prolonged exposure of cells to BrdU allows for the
identification and analysis of actively cycling, as opposed
to non-cycling, cell fractions. Pulse labeling of cells with
BrdU at various time points, permits the determination
of cell-cycle kinetics. BrdU incorporation studies have
been used in a variety of experimental protocols. These
include in vitro and in vivo (eg, intraperitoneal injection
or administration via drinking water) labeling systems.
注意事项：
The kits (Catalog Nos. 559619 and 552598) contain the
following components. Because some kit components are
stored at 4°C and others are stored at –80°C, the kit
components are shipped separately. See Storage and
handling (page 12) for details on storage.
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