描述:
The BD IMag™ Human T Lymphocyte Enrichment Set - DM is used for the negative selection of T lymphocytes from peripheral blood. The
Biotin Human T Lymphocyte Enrichment Cocktail contains monoclonal antibodies that recognize antigens expressed on erythrocytes,
platelets, and peripheral leukocytes that are not T lymphocytes. The BD IMag™ Streptavidin Particles Plus - DM are magnetic nanoparticles
that have streptavidin covalently conjugated to their surfaces. With these two components, the BD IMag™ Human T Lymphocyte Enrichment
Set - DM avoids the inadvertent activation of the enriched T lymphocytes by using reagents that do not directly bind to those T cells. This
Enrichment Set has been optimized for use with the BD IMagnet™, and it contains sufficient reagents to label 10^9 peripheral blood
mononuclear cells (PBMC).
The T Lymphocyte Enrichment Cocktail component is comprised of the following biotin-conjugated monoclonal antibodies:
Anti-human CD11b/Mac-1 (CR3), clone ICRF44
Anti-human CD16, clone 3G8
Anti-human CD19, clone HIB19
Anti-human CD36, clone CB38 (NL07)
Anti-human CD41a, clone HIP8
Anti-human CD56, clone B159
Anti-human CD235a (Glycophorin A), clone GA-R2 (HIR2)
注意事项:
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C.