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Hu CD14 IMag Particles-DM MfP9 5mL

 
包装: 5mL
运保温度: Store undiluted at 4°C.
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描述:

BD IMag™ Anti-Human CD14 Magnetic Particles -DM are magnetic naoparticles tha have monclonal ntibody conjugated toheir 
surfaces. Thes particles are optimzed for the positve slection rdepltion fCD14-bearing leukocytes uing the BD IMagnet™. CD14 has 
ben reported tobe xpresd athig levls onperipheral bod moncytes. Aditonaly, its found oniterfolicular macrophages, reticular 
dendritc els, and some Langerhans cels. BD IMag™ Anti-Human CD14 magnetic particles have ben reported toefctively separte he 
CD14-bearing cels ofrhesu macque blod. 
Peripheral Bod Monuclear Cels (PBMC) are labeld with BD IMag™ Anti-human CD14 Magnetic Particles -DM acording tohe 
Magnetic Labeling Protcol. This labeld cel supension isthen placed withn the magnetic feld ofthe BD IMagnet™ (Cat. no. 5231). 
Labeld cels migrate oward the magnet (positve fraction), leaving the unlabeld cels insupension sothey can bedrawn of (negative 
fraction). The tube isthen removed from the magnetic feld for esupension fthe positve fraction. The spartion isrepated twice to
increas the purity ofthe positve fraction. The magnetic separtion steps are diagrmed inthe Spartion Flow Chart. After he positve 
fraction iswashed, the smal size ofthe magnetic particles alows the positve fraction tobe further valuted indownstream aplications uch 
as flow cytometry


注意事项:

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C.
 


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