Ms Ly-49A B6 Biotin A1 100ug

• 产品编号：BD-557423      品牌：BD-Pharmingen       原厂货号：557423
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

The A1 antibody reacts with the Ly-49A[B6] alloantigen, an inhibitory receptor which is expressed on subsets of natural killer (NK) cells and
NK-1.1+ T lymphocytes (NK T cells) in C57BL/6, C57BL/10, and B10 congenic mice, on a population of memory CD8+ T lymphocytes and
NK1.1+ γδ T cells in C57BL/6 mice, and on a distinct subset of B-1 cells (CD5+B220[lo]) of C57BL/6 mice. A1 mAb has also been reported
to cross-react with Ly-49ANOD, Ly-49PNOD, Ly-49P129/J, and Ly-49V129/J alloantigens. The proportion of NK T cells expressing Ly-49A
is higher (2-5 fold) in thymus than in liver (immature and mature NK T cells, respectively), and there is evidence that the down regulation of
Ly-49 receptor expression is necessary for normal NK T-cell development to occur. Most NK cells express a single allele of Ly-49A, although
occasionally they may express more than one allele. The Ly-49 family of NK-cell receptors, members of the C-type lectin superfamily, are
disulfide-linked type-II transmembrane protein homodimerswith extracellular carbohydrate-recognition domains (CRD) that bind to MHC
class I alloantigens. The A1 antibody is specific for the Ly-49A[B6] CRD. The Ly-49 family members are expressed independently, such that
an individual NK or T cell may display more than one class of Ly-49 receptor homodimers. The Ly-49A[B6] allonantigen binds to H-2D[d],
H-2D[k], and H-2D[p], and the A1 antibody blocks this binding. Binding of Ly-49A[B6] to lyphoblasts expressing MHC class I antigens of
the f, q, r, s, and v haplotypes has also been demonstrated. The levels of the Ly-49 inhibitory receptors are down-regulated by their ligands in
vivo, and various levels of expression of a Ly-49 inhibitory receptor may affect the specificity of NK cells. In vitro studies suggest that the
Ly-49A receptor mediates negative regulation of NK-cell cytolytic activity via tyrosine phosphorylation of its ITIM (Immunoreceptor
Tyrosine-based Inhibitory Motif).

原厂资料：

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.