Hu SREBP-2 Pure IgG-1C6 100ug

• 产品编号：BD-557037      品牌：BD-Pharmingen       原厂货号：557037
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SREBP-1 and -2 (sterol-regulatory element binding proteins-1 and -2) are transcription factors which participate in the control of cholesterol
homeostasis. SREBP proteins, which are attached to the endoplasmic reticulum and nuclear envelope, are proteolytically cleaved and thus
activated in response to conditions of low cellular sterol. Upon activation of SREBP-1 or -2, a ~480-500 amino acid, N-terminal cleavage
fragment of these proteins enters the nucleus and activates transcription of enzymes and other proteins required for cholesterol synthesis.
Proteases which cleave SREBPs have been identified and include SCA (SREBP-cleavage activity), as well as caspase-3, a key regulator of
apoptotic pathways. SREBP proteins containing point mutations at caspase-3 cleavage sites (Asp460 in SREBP-1 and Asp468 in SREBP-2)
do not become cleaved following induction of apoptosis, suggesting that SREBPs may play some role in apoptotic processes. However,
sterol-regulated vs. apoptosis-associated cleavage of SREBP proteins appears to be independently regulated. On SDS-PAGE, sterol-regulated
cleavage fragments of SREBP proteins migrate more slowly (i.e., higher molecular weight) than do staurosporin-induced fragments. In
addition, staurosporin-induced SREBP cleavage products may appear as a doublet, with the upper band representing a phosphorylated form of
SREBP. On SDS-PAGE, full length, precursor forms of SREBP-1 and -2 migrate at ~125 kD, while proteolytic cleavage fragments may be
observed as a cluster of bands between 60 - 70 kDa.

原厂资料：

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C.