The 3G8 monoclonal antibody specifically binds to the 50-65 kDa transmembrane form of the IgG Fc Receptor (Fc γ RIII), a human NK cell-associated antigen. CD16 is expressed on NK cells as well as macrophages and granulocytes. Reports indicate that CD16 plays a role in signal transduction and NK cell activation. The 3G8 antibody blocks the binding of soluble immune complexes to granulocytes. The 3G8 antibody is reported (Vossebeld et al ., 1997) to increase intracellular calcium levels in human neutrophils by interacting with both Fc γ RIIa and Fc γ RIIIb molecules. This antibody has also been reported to induce homotypic neutrophil aggregation.
原厂资料：
The 3G8 monoclonal antibody specifically binds to the 50-65 kDa transmembrane form of the IgG Fc Receptor (Fc γ RIII), a human NK cell-associated antigen. CD16 is expressed on NK cells as well as macrophages and granulocytes. Reports indicate that CD16 plays a role in signal transduction and NK cell activation. The 3G8 antibody blocks the binding of soluble immune complexes to granulocytes. The 3G8 antibody is reported (Vossebeld et al ., 1997) to increase intracellular calcium levels in human neutrophils by interacting with both Fc γ RIIa and Fc γ RIIIb molecules. This antibody has also been reported to induce homotypic neutrophil aggregation.
This clone also cross-reacts with a subset of perripheral blood lymphocytes and monocytes, but not granulocytes, of baboon and both rhesus and cynomolgus macaque monkeys. Multi-color analysis reveals that the distribution on lymphocytes is similar to that found in human studies, with the majority of CD16-positive lymphocytes being both CD3 and CD20 negative.
注意事项：
1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
5.The antibody was conjugated with FITC under optimum conditions, and unconjugated antibody and free FITC were removed.
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