Hu DNA Topoisomerase I Pure C-21.2 100ug

• 产品编号：BD-556597      品牌：BD-Pharmingen       原厂货号：556597
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DNA damage may be caused by various environmental factors, including radiation, mutagenic chemicals and copy errors which occur during
DNA replication. The correction of DNA damage, so called "proofreading" functions of the cell, is achieved by numerous excision-repair
enzymes. Topoisomerases alter the helical structure of DNA by introducing the transient breaking and rejoining of DNA strands, allowing
other excision-repair enzymes to correct DNA errors. Topoisomerase I (Topo I) is a ubiquitous, soluble enzyme whose expression is fairly
constant throughout the cell cycle. The related enzyme, Topo II, is a primarily insoluble structural protein whose expression varies between
cell types and during the cell cycle. In addition to its role in DNA mismatch repair, Topo I displays kinase activity, phosphorylating
serine-arginine rich (SR) splicing factors, and perhaps regulating gene expression by changing the splicing pattern of structural genes. DNA
Topo I migrates at a molecular weight of 100 kDa in SDS-PAGE. Clone C-21 recognizes human DNA Topoisomerase I. The antibody is
routinely tested by western blot analysis of A-431 cell lysates.

原厂资料：

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C.