Hu Cdc25C Pure C2-2 100ug

• 产品编号：BD-556576      品牌：BD-Pharmingen       原厂货号：556576
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes.
Cyclins contain a conserved amino acid sequence motif, the cyclin box, through which they bind to cdks to form active complexes that
regulate the progression of the cell cycle. These complexes are regulated by activating and inhibitory phosphorylation events, as well as by
interactions with small regulatory proteins including p21 and p27Kip1. Cdc25A, Cdc25B and Cdc25C are a family of phosphatases that
activate cyclin-cdk complexes at different checkpoints of the cell cycle. Cdc25C appears to be active during G2 where it controls entry into
mitosis by dephosphorylating and thus activating the cdk1 component of cyclin B1-cdk1 complexes. In response to DNA damage, Cdc25C
becomes phosphorylated by the recently identified kinases Chk1 and c-TAK1 creating a binding site for 14-3-3 proteins which inhibits the
phosphatase activity of Cdc25 proteins. Thus Cdc25C serves a dual purpose during the cell cycle via its ability to promote G2/M transition in
normally cycling cells as well as by providing a cell cycle checkpoint in response to DNA damage.
Multiple electrophoretic forms of Cdc25C have been reported and are thought to result from differences in the phosphorylation state of the
Cdc25C protein. Full length, recombinant human Cdc25C was used as immunogen. HeLa human cervical carcinoma (ATCC CCL-2) and
A549 human lung carcinoma (ATCC CCL-185) cells are suggested as positive controls for the western blot application.

原厂资料：

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C.