Ac-IETD-AFC Casp-8 Flrgnc Sbstr Pure 1mg

• 产品编号：BD-556552      品牌：BD-Pharmingen       原厂货号：556552
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 多肽 > 功能肽
• 应用分类：

描述：

The caspase family of cysteine proteases was discovered following a search of human cDNA libraries for sequences homologous to ced-3, a
cell death gene described in the nematode worm C. elegans. The name caspase reflects the catalytic properties of these enzymes. The "c"
denotes their cysteine protease mechanism and "aspase" refers to their ability to cleave after aspartic acid residues. These proteases are
expressed as inactive proenzymes, which are proteolytically cleaved into large and small subunits, which form the active enzyme. Caspase-8
(FLICE/MACH-1) is a 55 kD cytosolic protein with homology to the CD95/Fas-associated signal transduction molecule, FADD, in addition to
its homology with other caspases. Caspase-8 is activated early in apoptosis and is involved in the proteolysis and activation of pro-caspase-3.
The upstream sequence of the site recognized by active caspase-8, IETD (Ile-Glu-Thr-Asp), is utilized as a basis for the highly specific
caspase-8 substrate, Ac-IETD-AFC, and the caspase-8 inhibitor, Ac-IETD-CHO. Ac-IETD-AFC is a synthetic tetrapeptide substrate for
caspase-8 and contains the amino acid sequence which is the target for caspase-8-mediated proteolysis. The tetrapeptide substrate can be used
to identify and quantify caspase-8 activity in apoptotic cell lysates and to study events downstream of caspase-8 activation.

原厂资料：

注意事项：

Store the lyophilized Ac-IETD-AFC substrate at -20°C. Reconstitute the Ac-IETD-AFC substrate with 1 ml DMSO before use.
Store the reconstituted Ac-IETD-AFC substrate at -20°C for up to two months and avoid repeated freeze-thaw cycles, which
can greatly alter product stability.