Hu Bcl-2 PE Set Bcl-2/100 100Tst

• 产品编号：BD-556535      品牌：BD-Pharmingen       原厂货号：556535
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

描述：

Programmed cell death (apoptosis) is a normal physiologic process which occurs during embryonic development as well as in maintenance of
tissue homeostasis. The apoptotic program is characterized by certain morphological features. These include changes in the plasma membrane
such as loss of membrane asymmetry and attachment, a condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA. In
the final stages, the dying cells become fragmented into “apoptotic bodies” which are rapidly eliminated by phagocytic cells without eliciting
significant inflammatory damage to surrounding cells. Members of the Bcl-2 family play a major role in regulating the response of cells to
apoptotic signals. Bcl-2 is considered to be novel among proto-oncogenes because it blocks apoptosis in many cell types. Bcl-2 is thought to
provide selective survival advantage for cells by blocking apoptosis and thus may contribute to tumorigenesis. Bcl-2 is a 26 kDa intracellular,
integral membrane protein found primarily in the nuclear envelope, endoplasmic reticulum and outer mitochondrial membrane.
Clone Bcl-2/100 reacts with human Bcl-2. It does not cross-react with mouse Bcl-2. A synthetic peptide corresponding to amino acids 41-54
(GAAPAPGIFSSQPG) of human Bcl-2 was used as immunogen. This peptide sequence is not conserved between human and mouse. Clone
Bcl-2/100 was initially characterized by western blotting and immunohistochemistry of frozen and paraffin-embedded tissue sections. Clone
MOPC-21 is a mouse IgG1 isotype control. The MOPC-21 antibody has unknown specificity.

原厂资料：

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
5.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.