Hu PARP Pure 4C10-5 100ug

• 产品编号：BD-556494      品牌：BD-Pharmingen       原厂货号：556494
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PARP [Poly(ADP-ribose) polymerase] is a 113 kDa nuclear chromatin-associated enzyme that catalyzes the transfer of ADP-ribose units from
NAD+ to a variety of nuclear proteins including topoisomerases, histones, and PARP itself. The catalytic activity of PARP is increased in
non-apoptotic cells following DNA damage, and PARP is thought to play an important role in mediating the normal cellular response to DNA
damage. Additionally, PARP is a target of the caspase protease activity associated with apoptosis. During apoptosis, PARP is cleaved from a
113 kDa intact form into smaller 89 kDa and 24 kDa fragments. This process separates the amino-terminal DNA-binding domain of the
enzyme from the carboxy-terminal catalytic domain resulting in the loss of normal PARP function. Although the role of PARP in apoptosis
remains to be elucidated, PARP cleavage is considered to be a marker of apoptosis. The 4C10-5 antibody recognizes both the intact 113 kDa
form and 89 kDa fragment of PARP.
The 4C10-5 antibody has been reported to recognize both native and denatured PARP. Purified human PARP was used as the immunogen and
the antibody reported to react with an epitope located in the NAD binding domain. In dot blot assays, the antibody reacts with the native
enzyme in the presence or absence of bound DNA as well as after synthesis of covalently linked poly (ADP-ribose). The 4C10-5 antibody is
routinely tested by western blot analysis of untreated Jurkat T cells and Jurkat T cells induced to undergo apoptosis.

原厂资料：

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C.