Caspase-3 Assay Kit

• 产品编号：BD-556485      品牌：BD-Pharmingen       原厂货号：556485
• 产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞凋亡试剂盒
• 应用分类：

其它组分：

描述：

This kit is designed to measure caspase-3 or DEVD-cleaving activity, an early marker of cells undergoing apoptosis. The kit can also be used
to determine if certain apoptosis pathways involve caspase-3 activation. It contains a caspase-3 fluorogenic substrate (Ac-DEVD-AMC), a
caspase-3 aldehyde inhibitor (Ac-DEVD-CHO) and assay buffers.
Ac-DEVD-AMC is a synthetic tetrapeptide fluorogenic substrate (M.W. = 675 Daltons; purity ≥98%) that is used to identify and quantitate
caspase-3 activity in apoptotic cell lysates. Ac-DEVD-AMC has been reported to have linear Michaelis-Menton kinetics with a Km of 10 μM
for caspase-
3. Caspase-3 cleaves the tetrapeptide between D and AMC, releasing the fluorescent AMC which can be quantified in cell lysates by
ultraviolet (UV) spectrofluorometry using an excitation wavelength of 380 nm and an emission wavelength range of 420-460 nm. Apoptotic
cell lysates containing active caspase-3 yield a considerable emission as compared to non-apoptotic cell lysates (Fig. 1).
Ac-DEVD-CHO is a potent, synthetic tetrapeptide inhibitor (M.W. = 502.5 Daltons; purity ≥98%) of caspase 3 activity. The inhibitor is used
to block caspase-3 activity in apoptotic cells or cell lysates (Fig. 1). Ac-DEVD-CHO has been reported to have linear Michaelis-Menton
kinetics with a Ki of less than 1 nM for Caspase-3. This Ki (1 nM) is significantly less than the Km of the Ac-DEVD-AMC substrate (10 μM).
Thus, the amount of Ac-DEVD-CHO required for caspase-3 assays is much less than that of Ac-DEVD-AMC. When apoptotic cell lysates are
incubated with both substrate and inhibitor, caspase-3 activity is blocked by Ac-DEVD-CHO. As a result, AMC is not released from
Ac-DEVD-AMC and fluorescence is not emitted upon excitation of the samples.

原厂资料：

注意事项：

The Caspase-3 Kit contains two parts (A and B), which are shipped separately. Part A (Cat. No. 51-6632AK) is stored at 4°C
and shipped at room temperature. Part B (Cat. No. 51-6632BK) is stored at -20°C and shipped on dry ice. We have determined
that this shipping method is adequate to preserve kit integrity. Upon arrival, immediately store products at their appropriate
storage conditions.