热门搜索： A3500　　E1910　　A1360　　25200056　　dmem　　

Hu Active Caspase-8 Enzyme Pure 5ug

包装：

5ug

运保温度:

Store product at -80°C prior to use or for long te

到货周期：

登录后查看

标准价：

￥客户可见

会员价：

￥客户可见

积分：

客户可见分

登录之后可加入购物车购买，请您

运费与支付说明：

1.含干冰类产品有运费；

2. 必须现金付款或有信用额度的会员才可以直接发货，否则需要等待现金付款信息。

描述：

Caspases are cysteine proteases that play a central role in apoptosis. The caspase family was discovered by searching human cDNA libraries

for sequences homologous to ced-3, a C. elegans death gene that is required for normal apoptosis during development. The first mammalian

homolog of ced-3 to be identified was ICE (interleukin-1β-converting enzyme). Subsequent numerous human ced-3 homologues were rapidly

identified which led to multiple names for many of the molecules. To achieve consistency, "caspase" was adopted as a root name for all family

members. The name was selected based on two catalytic properties of these enzymes, the "c" reflects a cysteine protease mechanism and

"aspase" refers to their unique ability to cleave after aspartic acid. There are at least 10 members, caspase-1 (ICE), caspase-2 (ICH-1),

caspase-3 (CPP32, Yama, apopain), caspase-4 (TX, ICH-2, ICErel-II), caspase-5 (ICErel-III), caspase-6 (Mch2), caspase-7 (Mch3,

ICE-LAP3, CMH-1), caspase-8 (MACH, FLICE, Mch5), caspase-9 (ICELAP6, Mch6), and caspase-10 (Mch4). Each caspase is synthesized

as an inactive proenzyme that is processed by cleavage at asparte residues by another protease or by self-proteolysis. The processed forms

consist of large (17-22 kDa) and small (10-12 kDa) subunits which associate to form an active enzyme. The activation of some of these

caspases has been shown to occur during apoptosis.

Caspase-3, -6, -7, and -8 have been shown to play a role in apoptosis induced by the death receptors Fas and tumor necrosis factor receptor

type 1 (TNFR1). One of their substrates is poly (ADP ribose) polymerase (PARP). PARP is an enzyme that is involved in DNA repair and

genomic maintenance. Activated caspases 3, 6, 7 and 8 can all cleave PARP from its 116 kDa form to an 85 kDa residual fragment. The

cleavage separates the DNA-binding domain in the N-terminus of PARP from its C-terminus catalytic domain, and results in loss of normal

PARP function. The cleavage site in PARP is C-terminal to Asp-216.3 The upstream sequence of the PARP cleavage site, DEVD

(Asp-Glu-Val-Asp), is utilized as a basis for highly specific caspase-3 substrates such as Ac(N-acetyl)-DEVD-AFC

(7-amino-4-trifluoromethylcourmarin) and Ac(N-acetyl)-DEVD-AMC (7-amino-4-methylcoumarin) as well as the caspase-3 aldehyde

inhibitor Ac-DEVD-CHO.

注意事项：

Store product at -80°C prior to use or for long term storage of stock solutions.

The thawed active enzyme is stable at 4°C for at least a week.

Avoid multiple freeze-thaw cycles and exposure to frequent temperature changes, which can greatly alter product stability