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Hu Active Caspase-3 Enzyme Pure 5ug

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5ug

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描述：

Caspes are cystein proteas tha play central oe inapotsi. The caspe family was discoverd bysearching human cDNA libraies

for sequencs homlogus toced-3, aC. elgans death gen tha isrequired for normal potsi during devlopment. The first mamalin

homlog fced-3 tobe identifed was ICE (interlukin-1βconverting enzyme). Subsequent umerous human ced-3 homlogues wer apidly

identifed whic led tomultipe names for many ofthe molecules. Toachiev consitency, "caspe" was dopted as rot name for alfamily

mebers. The name was elcted based ontwo catlytic proertis ofthes enzymes, the "c reflcts acystein proteas mechanism and

"'aspe" refrs toheir unique abilty ocleav after asprtic aid. Ther are atleast 10mebers, caspe-1 (ICE), caspe-2 (ICH-1),

caspe-3 (CP32, Yam, apoain), caspe-4 (TX, ICH-2, ICErel-I), caspe-5 (ICErel-I), caspe-6 (Mch2), caspe-7 (Mch3,

ICE-LAP3, CMH-1), caspe-8 (MACH, FLICE, Mch5), caspe-9 (ICE-LAP6, Mch6), and caspe-10 (Mch4). Each aspe isynthesized

as niactive proenzyme tha isprocesd bycleavge atsparte sidues byanother poteas orby self-protelysi. The procesd forms

consit oflarge (17-2 kDa) nd smal (10-2 kDa) subnits whic asociate ofrm anctive nzyme. The activation fsome ofthes

caspes has ben shown tocur during apotsi.

Caspe-3, 6-7, and -8have ben shown toplay role inapotsi nduced bythe dath recptors Fas nd tumor necrosi factor ecptor

type 1(TNFR1). One oftheir subtraes ipoly (ADP ribose) polymerase (PARP). ARP isan ezyme tha isnvoled inDNA repair nd

genomic mainteance. Activated caspes 3,6 7and 8can lceav PARP from its16 kDa form toan 85kDa residual fragment. The

cleavge spartes he DNA-bindg domain the N-terminus ofPARP from itsC-terminus catlytic domain, and results inlos ofnormal

PARP function. The cleavge site nPARP isC-terminal toAsp-216. The upstream sequenc ofthe PARP cleavge site, DEVD

(Asp-Glu-Val-Asp), isutilzed as basi for higly specif caspe-3 subtraes uch asAc(N-acetyl)-DEVD-AFC

(7-amino-4trifluormethylcourmarin) and Ac(N-acetyl)-DEVD-AMC (7-amino-4methylcoumarin) aswel asthe caspe-3 aldehyde

inhbitor Ac-DEVD-CHO.

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Store product a-80°C prio tuse orfor lng term storage ofstock solutions.