描述：
The Bcl-2 family members are involved in mediating programmed cell death or apoptosis, and share two highly conserved functional regions,
Bcl-2 homology 1 and 2 (BH1 and BH2). Several of the family members including Bcl-2 act as inhibitors of apoptosis, whereas others such as
Bax promote cell death. It is thought that protein-protein interactions between Bcl-2 family members play an important role in their function.
For example, Bax may form homodimers or heterodimers with either Bcl-2 and Bcl-XL (long). Bax homodimers is thought to promote cell
death, whereas Bax heterodimerization with either Bcl-2 and Bcl-XL appears to block cell death. When Bax is present in excess, it can
counteract the ability of Bcl-2 to inhibit cell death. Bax expression has been identified in a variety of tissues, including lung, stomach, kidney,
thymus, lymph nodes, bone marrow, spleen, heart, exocrine pancreas, and brain. Liver, kidney, and the exocrine pancreas have been found to
contain little or no Bcl-2. The Bax gene potentially encodes three different proteins Bax-α (21 kDa), Bax-β (24 kDa) and Bax-γ (5 kDa). Bax-α
is the most common transcript.
The 6A7 antibody recognizes human, mouse and rat Bax. The 6A7 antibody reacts with an epitope between amino acids 12-24 of Bax, and
studies suggest that this epitope may be in the vicinity of the dimerization domain of Bax. It has been proposed that the 6A7 antibody may
directly compete with either Bcl-2 or Bcl-XL for binding to Bax. In western blots, the 6A7 antibody typically detects Bax as a single band of
21-22 kDa, thought to be Bax-α.
注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C.
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