Propidium Iodide Staining Solution 2mL

产品编号：BD-556463 品牌：BD-Pharmingen 原厂货号：556463
产品分类：生化试剂 > 染料/染色剂/指示剂/底物 > 细胞染料 > 细胞核酸染料
应用分类：核酸分离纯化与分析 > 核酸凝胶电泳与印迹 > DNA染料

包装：

2mL

运保温度:

Store undiluted at 4° C

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描述：

碘化丙啶（PI）染色液可用于膜联蛋白V凋亡试验中检测细胞膜（PM）的完整性。PI是一种可使DNA染色的荧光活体染料。因为细胞处于存活状态或凋亡的早期阶段，细胞膜保持完整，所以PI不能穿过细胞膜。与之相反，细胞凋亡后或已经处于死亡的后期阶段，细胞膜失去完整性，PI可透过细胞膜。使用562-588纳米的橙色波长范围内带，可检测到PI的荧光。细胞的早期细胞凋亡阶段，膜联蛋白V即可结合到细胞并继续保持结合状态直至细胞死亡。PI可用于膜联蛋白V双染FACS分析，以区分凋亡早期的细胞（膜联蛋白V阳性，PI阴性）和处于凋亡的后期阶段或已经死亡（膜联蛋白V阳性，PI正）的细胞。

原厂资料：

The Prodidium Iodide (PI) Staining Solution may be used to assess plasma membrane (PM) integrity in Annexin V apoptosis assays. PI is a fluorescent vital dye that stains DNA. It does not cross the PM of cells that are viable or in the early stages of apoptosis because they maintain PM integrity. In contrast those cells in the late stages of apoptosis or already dead have lost PM integrity and are permeable to PI. PI is detected in the orange range of the spectrum using a 562-588 nm band pass filter. Annexin V binds to cells early in apoptosis, and continues to be bound through cell death. PI is used in two-color Annexin V flow cytometric assays to distinguish cells that are in the earlier stages of apoptosis (Annexin V positive, PI negative) from those that are in the later stages of apoptosis or already dead (Annexin V positive, PI positive).

注意事项：

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).

3.Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.