Perforin has a key role in cell-mediated cytotoxicity. It is a 70 kDa cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic
T lymphocytes (CTLs) and natural killer (NK) cells. CTLs are involved in eliminating virally infected cells, in anti-tumor immune responses,
in allograft rejections, and in some autoimmune diseases. NK cells are important for tumor surveillance and destruction and are involved in
allograft rejections. Cytotoxic cells release the contents of their cytotoxic granules, including perforin upon recognition of their target cell. In
the presence of calcium, perforin forms transmembrane channels or pores in the membrane of the target cell leading to a cell death that
resembles apoptosis. The ability to detect perforin-positive cells with specific antibody should be useful in identifying and understanding
perforin-mediated reactions.
Clone δG9 reacts with human and bovine perforin. It does not cross-react with mouse perforin. Purified granules from the human lymphoma
cell line YT were used as immunogen. Clone δG9 was initially characterized by immunoprecipitation and immunohistochemistry of frozen
tissue sections. The antibody stains scattered lymphocytes in red pulp of spleen, and scattered infiltrated lymphocytes in lymphoma.
原厂资料：
注意事项：
1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
5.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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