描述：
A cytochrome is an electron-transporting protein that contains a heme prosthetic group. Cytochromes have been known to be essential
components of the mitochondrial respiratory chain since 1925. The iron atom of the heme group in cytochromes alternates between a reduced
ferrous (+2) state and an oxidized ferric (+3) state during electron transport in oxidative phosphorylation. Cytochromes are classified into four
groups (a, b, c and d) according to spectrochemical characteristics, and there are five cytochromes between coenzyme QH2 and O2 in the
electron transport chain. Cytochrome c is a water-soluble protein that either promotes cell survival or death, depending upon its intracellular
location. In healthy cells, it is a peripheral membrane protein of the mitochondria that transports electrons from the coenzyme QH2
cytochrome c reductase complex to the cytochrome c oxidase complex. When proapoptotic stimuli induce breakdown of the mitochondria,
cytochrome c is released to the cytosol where it functions in the activation of caspases that trigger apoptosis.
The 6H2.B4 monoclonal antibody has been reported to recognize the native and not the denatured form of rat, mouse, and human cytochrome
c. Furthermore, studies utilizing competitive ELISA indicate that mAb 6H2.B4 binds to a region around residue 62 of rat cytochrome c.
The 6H2.B4 monoclonal antibody is not useful for western blot analysis. For the western blot application, clone 7H8.2C12 (cat.no. 556433)
would be recommended. Suggested positive controls for detecting cytochrome c include P388D mouse lymphoma cells (ATCC CCL-46),
HeLa human carcinoma cells (ATCC CCL-2), Jurkat T leukemia cells (ATCC TIB-152) and NIH 3T3 mouse fibroblast cells (ATCC
CRL-1658). This antibody is routinely tested by immunoprecipitation. Other applications were tested at BD Biosciences Pharmingen during
antibody development only or reported in the literature.
注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C.
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