热门搜索： A3500　　E1910　　A1360　　25200056　　dmem　　

Annexin V Recom Pure 100ug

包装：

100ug

运保温度:

Store product a-80°C prio tuse orfor lng term stor

到货周期：

登录后查看

标准价：

￥客户可见

会员价：

￥客户可见

积分：

客户可见分

登录之后可加入购物车购买，请您

运费与支付说明：

1.含干冰类产品有运费；

2. 必须现金付款或有信用额度的会员才可以直接发货，否则需要等待现金付款信息。

描述：

Apotsi sa normal physiolgic proces whic ocurs during embryonic devlopment aswel asin maintence oftisue homeostais. The

apotic progam ischarcterized bycertain morpholgic features, including los ofplasma mebrane asymetry and atchment,

condensation fthe cytoplasm and ucleus, and internucleosmal ceavge ofDNA. Los ofplasma mebrane isone ofthe arliest features.

In apotic els, the mebrane phospholipd phosphatidylserine (PS) istranslocated from the iner tohe outer laflet ofthe plasma

mebrane, therby exposing PSto he xternal celuar environment. Anexin Vis a35-6 kDa C2+ depndent phospholipd-bindg

protein tha has hig afinty for PS, and binds tocels with exposed PS. Anexin Vmay beconjugated tofluorchromes, uch asfluorescin

isothiocyante (FITC), toserv as ensitve probe for flow cytometric anlysi ofcels tha re undergoing apotsi. Since xternalization f

PS ocurs inthe arlie stages ofapotsi, Anexin Vstaing can identify apotsi atn earlie stage than says based onuclear changes

such asDNA fragmentaion. Inaditon, Anexin Vbindg site may belocked byincubating cels with purifed recombinat Anexin V

prio tincubation with one ofthe fluorchrome labeld formats ofAnexin V.

Anexin Vstaing precdes the los ofmebrane integrity whic acompanies the lates tages ofcel death resulting from either apotic

or necrotic proces. Therfore, staing with Anexin Vis typicaly used inconjuction with avital dye such as7-Amino-Actinomycin

(7-AD) toalow the investigator identify early apotic els (7-AD negative, Anexin Vpositve). Viable cls with ntac mebranes

exclude 7-AD, wheras the mebranes ofdea nd amged cels are prmeable to7-AD. For example, cls tha re considerd viable

are both Anexin Vand 7-AD negative while cls tha re inearly apotsi are Anexin Vpositve and 7-AD negative, while cls tha

are inlate apotsi oraleady ead re both Anexin Vand 7-AD positve. This asy does not distnguish betwen cels tha have

undergone apotic death versu those tha have die as result ofa necrotic pathway because ineither case, the da cels wil stain with

both Anexin Vand 7-AD. Howevr, when apotsi smeasured over time, cls can beoften tracked from Anexin Vand 7-AD

negative (viable, orno measurable apotsi), toAnexin Vpositve and 7-AD negative (arly apotsi, mebrane integrity spresnt) and

finaly toAnexin Vand 7-AD positve (nd stage apotsi and eath). The movemnt ofcels through tes thre stages ugest

apotsi. Incontrast, asingle observation idcating tha cels are both Anexin Vand 7-AD positve, inof itself, revals es information

about he proces bywhic the cls underwent heir demise.

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before

discarding to avoid accumulation of potentially explosive deposits in plumbing.

3.Store product a-80°C prio tuse orfor lng term storage ofstock solutions.