The 2D7/CCR5 monoclonal antibody specifically binds to the human chemokine receptor, CCR5. CCR5 is also known as CD195. CCR5 is a
seven transmembrane-spanning G protein-associated molecule. CCR5 belongs to the beta chemokine receptor family. It is expressed on a
subset of T lymphocytes (CD3+CD45RO+CD95+). CCR5 regulates lymphocyte chemotaxis activation and transendothelial migration during
inflammation. It signals a response to at least three chemokines: Regulated upon Activation Normal T-cell Expressed and Secreted
(RANTES), Macrophage Inflammatory Protein-1 (MIP-1) and Monocyte Chemoattractant Protein 2 (MCP-2). Additionally, CCR5 has been
found to be a coreceptor for macrophage-tropic HIV-1 on CD4+ cells, a characteristic that is important in viral transmission. Reports indicate
that individuals who have partial (heterozygous) or complete (homozygous) deletion of the CCR5 allele, demonstrate resistance to HIV
infection. This antibody has been shown to block ligand and gp120 binding. It is also able to neutralize HIV infection.
原厂资料:
注意事项:
1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
5.The antibody was conjugated with FITC under optimum conditions, and unconjugated antibody and free FITC were removed.