Via-Probe Cell Viability Solution 500Tst

包装：

500Tst

运保温度:

Store undiluted at 4°C

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描述：

BD Via-Probe™ is a convenient, ready-to-use solution of the nucleic acid dye, 7-Amino-actinomycin D (7-AAD) that can be used in place of

propidium iodide (PI) for the exclusion of nonviable cells in flow cytometric assays. The advantage of 7-AAD over PI is the ability to be used

in conjunction with phycoerythrin (PE)- and fluorescein (FITC)-labeled monoclonal antibodies in 2-color analysis, with minimal spectral

overlap between the 7-AAD, PE and FITC fluorescence emissions. BD Via-Probe™ fluorescence is detected in the far red range of the

spectrum (650 nm long-pass filter). This reagent is used as a viability probe for methods of dead cell exclusion, based on light scatter and

uptake of 7-AAD as detected in FL3. This reagent does not require dilution, use 20 µL/test (1x10^6 cells) and incubate for 10 minutes before

analysis.

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental

sample (a test).

2.Since applications vary, each investigator should titrate the reagent to obtain optimal results..

3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before

discarding to avoid accumulation of potentially explosive deposits in plumbing.

4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.