Reacts with the α subunit (32 kDa) of the two-chain complex. CD8 molecule binds to HLA class I molecules during interaction of CD8+ T cells with antigen-presenting cells or with target cells. CD8 is expressed on T cytotoxic/suppressor cell populations. HIT8a stains approximately 13 - 48% of peripheral blood lymphocytes and 80% of thymocytes, as well as a subset of NK cells. Clones HIT8a and RPA-T8 (Cat. No. 555364) are not cross-blocking.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
原厂资料：
Reacts with the α subunit (32 kDa) of the two-chain complex. CD8 molecule binds to HLA class I molecules during interaction of CD8+ T cells with antigen-presenting cells or with target cells. CD8 is expressed on T cytotoxic/suppressor cell populations. HIT8a stains approximately 13 - 48% of peripheral blood lymphocytes and 80% of thymocytes, as well as a subset of NK cells. Clones HIT8a and RPA-T8 (Cat. No. 555364) are not cross-blocking.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
注意事项：
1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
5.The antibody was conjugated with FITC under optimum conditions, and unconjugated antibody and free FITC were removed.
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