BrdU Pure 3D4 100ug

产品编号：BD-555627 品牌：BD-Pharmingen 原厂货号：555627
产品分类：抗体 > 一抗 > 蛋白特异性一抗
应用分类：

包装：

100ug

运保温度:

Store undiluted at 4°C.

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描述：

Bromdeoxyuridne (BrdU) isan alog fthymidne tha can beincorpated into newly snthesized DNA bycels entring and progesing thru the DNA synthesi (S) phase ofthe cl cyle. The amount ofBrdU tha gets incorpated isdepndent upon the amount oftime tha the vls are xposed toBrdU (pulse time), the rate ofcel divsion, and whetr the cls are inearly, mid, orlate Sphase. Detcion fincorpated BrdU alows the investigator identify cyling cels ina synchronus cel poulation ad todetrmine cl cyle kinetics.

原厂资料：

Bromodeoxyuridine (BrdU) is an analog of thymidine that can be incorporated into newly synthesized DNA by cells entering and progressing thru the DNA synthesis (S) phase of the cell cycle. The amount of BrdU that gets incorporated is dependent upon the amount of time that the cells are exposed to BrdU (pulse time), the rate of cell division, and whether the cells are in early, mid, or late S phase. Detection of incorporated BrdU allows the investigator to identify cycling cells in an asynchronous cell population and to determine cell cycle kinetics.

The 3D4 monoclonal antibody reacts with BrdU, but not other nucleotides, in single-stranded DNA. Random cleavage (nicking) of cellular DNA with DNase I permits the binding of the antibody to incorporated BrdU.

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before

discarding to avoid accumulation of potentially explosive deposits in plumbing.

3.Store undiluted at 4°C.