Hu CD8 FITC RPA-T8 100Tst

• 产品编号：BD-555366      品牌：BD-Pharmingen       原厂货号：555366
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

RPA-T8单克隆抗体特异性地结合CD8α。CD8α是I型跨膜糖蛋白，是免疫球蛋白超家族中的一员。多数胸腺细胞，αβT细胞和γδT细胞的亚群和一些NK细胞均表达CD8α。CD8α有两种表达形式：二硫键连接的二聚体（CD8αα）或异二聚体（CD8αβ）。CD8阳性αβT细胞同时表达CD8αα同型二聚体和异二聚体CD8αβ，而有些γδT细胞和NK细胞表达CD8αα同二聚体。CD8在T细胞的活化和选择过程中起重要作用。 CD8α的胞外IgSF域结合于HLA I类分子（α3结构域）的一个非多态决定簇，使CD8在T细胞识别抗原的过程中成为MHC I类限制性的TCR的共同受体。CD8α的胞内结构域与Lck偶联。RPA-T8和HIT8a单克隆抗体没有交叉阻断作用。有报道表明，本抗体可与和狒狒、恒河猴和食蟹猕猴的外周血淋巴细胞的亚群发生反应，但不与单核细胞和粒细胞发生反应。通常，观察在非人类的灵长类动物与人类相比，CD8+和CD4+ CD8 +淋巴细胞的密度更大。

原厂资料：

The RPA-T8 monoclonal antibody specifically binds to CD8 alpha (CD8α). CD8α is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. CD8α is expressed by the majority of thymocytes, by subpopulations of αβ T cells and γδ T cells and by some NK cells. Cell surface CD8α is expressed either as a disulfide-linked homodimer (CD8αα) or as a heterodimer (CD8αβ) when disulfide-bonded to a CD8 beta chain (CD8β). CD8-positive αβ T cells coexpress both CD8αα homodimers and CD8αβ heterodimers whereas some γδ T cells and NK cells express CD8αα homodimers. CD8 plays important roles in T cell activation and selection. The extracellular IgSF domain of CD8α binds to a non-polymorphic determinant on HLA class I molecules (α3 domain) and enables CD8 to function as a co-receptor with MHC class I-restricted TCR during T cell recognition of antigen. The cytoplasmic domain of CD8α associates with Lck, a Src family protein tyrosine kinase that is involved in intracellular signaling. The RPA-T8 and HIT8a monoclonal antibodies are not cross-blocking. This clone has been reported to react with a subset of peripheral blood lymphocytes, but not monocytes nor granuloyctes, of baboon and both rhesus and cynomolgus macaque monkey. In general, a higher frequency of CD8+ and CD4+CD8+ lymphocytes are observed in non-human primates compared to normal human donors.

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental sample (a test).
2. An isotype control should be used at the same concentration as the antibody of interest.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
5. Species testing during development may have been performed with a different format of the same clone. Selected applications have been tested for cross-reactivity.
6.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.