The RPA-T4 clone reacts with CD4, a 59 kDa single-chain transmembrane glycoprotein [receptor for human immunodeficiency virus (HIV)]
present on T-helper/inducer cell populations. This antibody binds to the D1 domain (CDR1 and CDR3 epitopes) of the CD4 antigen and
reacts with approximately 80% of thymocytes and 45% of peripheral blood lymphocytes. CD4 is also present in low density on peripheral
blood monocytes. RPA-T4 is capable of blocking HIV-1, gp120, and inhibits syncytium formation.
原厂资料:
注意事项:
1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
5.The antibody was conjugated with APC under optimum conditions, and unconjugated antibody and free APC were removed.