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HiCK-1 Cytokine Positive Control Cells

 
包装: Cells
运保温度: Store product at -80°C prior to use or for long te
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描述:

This suspension contains Human intracellular CytoKine-1 Positive Control Cells (HiCK-1). The HiCK-1 frozen cell suspension contains 
fixed, non-permeabilized human lymphoid cells. The suspension includes cells that express detectable levels of intracellular IL-2, IFN-γ and 
TNF as determined by immunofluorescent intracellular cytokine staining and flow cytometry. HiCK-1 cell suspensions were prepared by 
stimulating human PBMCs in the presence of a protein transport inhibitor. After stimulation, the cells were harvested and fixed, then stored in 
1ml of 10% dimethylsulfoxide and 90% fetal bovine serum at -80°C. HiCK-1 cells contain a measurable proportion of cytokines, with 
representative flow cytometric data shown below. Performance from individual lots of HiCK-1 cells may differ due to donor variability. 
Investigators should anticipate similar, though not identical, results to those shown below. 


注意事项:

Store product at -80°C prior to use or for long term storage of stock solutions.
Rapidly thaw and quick-spin product prior to use.
Avoid multiple freeze-thaws of product.
This preparation contains no preservatives, thus it should be handled under aseptic conditions.
 


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