The Ha2/5 monoclonal antibody specifically binds to the 130 kDa integrin β1 chain (CD29). CD29 is expressed on the cell surface as a
heterodimer with one of the distinct integrin α chains. With α1 through α6 (CD49a through CD49f), it forms the VLA-1 through VLA-6
complexes, respectively, and with αV (CD51), it forms αVβ1 integrin. As a result, CD29 has a broad tissue distribution, including
lymphocytes, endothelia, smooth muscle, and epithelia. The Ha2/5 hamster anti-rat CD29 monoclonal antibody cross-reacts with mouse
thymocytes, splenocytes, and peripheral lymph node leukocytes. The Ha2/5 antibody blocks in vitro adhesion of CD29-expressing cells to
collagen.
原厂资料:
注意事项:
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
4.The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
5.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.