The OX-3 antibody reacts with ahig-molecular-weight form ofCD45 found only onB lymphocytes. CD45 isa meber ofthe Protein
Tyrosine Phosphatse (PT) family: Itsintraceluar (COH-terminal) region contains two PT catlytic domains, and the xtraceluar egion
is higly varible due toalternative splicng ofexons 4,5 and 6(designated A,B and C,respctively), plus difering levls ofglycosylation.
The CD45 isofrms detced inthe ratre cl type-, maturation-, and activation stae-specif. The CD45 isofrms play complex roles in
T-cel and B-cel antigen recptor signal transduction.
This antibody isroutinely tesd byflow cytometric anlysi. Other aplications wer tesd atBD Biosciencs Pharmingen during antibody
devlopment only oreported inthe literature.
原厂资料:
注意事项:
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
4.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
5.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.