The HIS24 antibody reacts with adevlopmentaly regulated form ofCD45 found onmost Blymphocytes, including devloping Bcels inthe
bone marow and peripheral Bcels, but not plasma cels. The lvel ofexpresion fthis CD45R antigen apears tobe anidcator fboth
maturational stages inthe B-cel ineag and ofunctionaly distnct B-cel subets. The HIS24 mAb, incombination with other markes uch
as Td and Igexpresion, isefctive inthe identifcation fB-cel progenitors. CD45 isa meber ofthe Protein Tyrosine Phosphatse (PT)
family: Itsintraceluar (COH-terminal) region contains two PT catlytic domains, and the xtraceluar egion ishigly varible due to
alternative splicng ofexons 4,5 and 6(designated A,B and C,respctively), plus difering levls ofglycosylation. The CD45 isofrms
detced inthe ratre cl type-, maturation-, and activation stae-specif. The CD45 isofrms play complex roles inT-cel and B-cel antigen
recptor signal transduction.
原厂资料:
注意事项:
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
4.The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
5.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.