Rat CD8a Biotin OX-8 500ug

The OX-8 antibody reacts with e hinge-like mebrane-proximal domain ofthe 32kDa αchain ofthe CD8 diferntiaon atigen. A

truncated CD8 α'isofrm has not ben detced inthe rat. The CD8 αand βchains (CD8a nd CD8b, respctively) form ahetrodimer onthe 

surface ofmost hymocytes and asubpoulation fmature Tlymphocytes (i.e, MHC clas I-restriced Tcels, including most T

supresor/cytoxic els). Intesinal intrapithelia lymphocytes, many CD8+ Tcels ofathymic rats, many activated CD4+ Tcels, and most 

NK cels expres CD8a without CD8b. Ithas ben sugestd tha the xpresion fthe CD8a/CD8b hetrodimer isrestriced to

thymus-derived Tlymphocytes. OX-8 antibody oes not react with resting CD4+ Thelpr cels. CD8 isan tigen corecptor nthe T-cel 

surface whic interacts with MHC clas Imolecules onatigen-presntig cels. Itparticpates inT-cel activation through itsasociaton with 

the T-cel recptor complex and protein tyrosine kinase Ick. Macrophages have also ben reported toexpres CD8 αand βchains, whic are 

involed insignal transduction. Solube OX-8 mAb partily blocks invitro MLR and CTL activy. 

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

3.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

4.The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

5.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.