The ex vivo addition of BD GolgiStop™, a protein transport inhibitor containing monensin, to in vitro- or in vivo-stimulated lymphoid cells blocks their intracellular protein transport processes. This results in the accumulation of cytokines and/or proteins in the Golgi complex. The increased accumulation of cytokines in the cell enhances the detectability of cytokine-producing cells with flow cytometric analysis.
Investigators should note that the appearance of BD GolgiStop™ may range in color from clear (colorless) to a light yellow.
注意事项:
Danger: BD GolgiStop™ Protein Transport Inhibitor, containing monensin (component 51-2092KZ) contains 99.61% ethanol (w/w) and 0.26% monensin, mononatriumsalz (w/w).
Hazard statements:
Highly flammable liquid and vapor.
Causes serious eye irritation.
Precautionary statements:
Keep away from heat/sparks/open flames/hot surfaces. No smoking.
Wear protective gloves / eye protection.
Wear protective clothing.
IF ON SKIN (or hair): Remove / Take off immediately all contaminated clothing. Rinse skin with water / shower.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Dispose of contents / container in accordance with local / regional / national / international regulations.