BD Perm/Wash™ Buffer, 10 × concentrate containing FBS* and saponin【包装:100ml】
BD GolgiStop protein transport inhibitor containing monensin【子货号:554724,包装:0.7ml】
描述:
本试剂盒提供包含莫能菌素的蛋白质转运抑制剂。并提供了足够处理1升体积的细胞培养物(细胞密度达2 X 10^6/ml)的反应试剂。
原厂资料:
In addition to the Fixation/Permeabilization solution and BD Perm/Wash™ Buffer included in the BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit, the BD Cytofix/Cytoperm Plus Fixation/Permeabilization Kit provides BD GolgiStop protein transport inhibitor containing monensin. The ex vivo addition of BD GolgiStop™ to in vitro- or in vivo-stimulated cells blocks their intracellular transport processes. This results in the accumulation of most cytokine proteins in the Golgi complex and thereby enhances cytokine staining signals See figures 2-4). Enough BD GolgiStop reagent is provided for treating up to 1 liter of cell culture at a cell density of up to 2 × 106 cells/ml.
注意事项:
The kit described in this product manual contain Fixation/Permeabilization solution containing 4% paraformaldehyde, and either BD GolgiStop™ protein transport inhibitor containing 99.9% ethanol, or BD GolgiPlug™ protein transport inhibitor containing 99.9% dimethylsulfoxide and 0.1% brefeldin A.
The following warnings and precautions apply to the aforementioned chemicals.
R 36/38 Irritating to eyes and skin S3 Keep in a cool place
S26 In case of contact with eyes, rinse immediately with water and seek medical advise
S28 After contact with skin, wash immediately with plenty of water
R40 Limited evidence of a carcinogenic effect
S36/37 Wear suitable protective clothing and gloves
* Source of all serum proteins is from USDA inspected abattoirs located in the United States.