Stain Buffer BSA 500mL

• 产品编号：BD-554657      品牌：BD-Pharmingen       原厂货号：554657
• 产品分类：生化试剂 > 免疫分析试剂 > 流式细胞术溶液和试剂
• 应用分类：

描述：

用于单细胞悬液的免疫荧光染色，样本无论来源于淋巴组织，骨髓，外周血或者细胞培养物均可适用。在荧光试剂的稀释、染色，以及流式分析试剂重悬、清洗、保存的过程中均可使用。其中的BSA成分可保持细胞活力，有助于pH敏感的荧光染料（如FITC）保持高强度信号。另一个组分叠氮化钠则代谢抑制剂，抑制细胞表面抗原的重组而导致的交叉反应及信号损失。

原厂资料：

PharmingenStain Buffer (BSA) can be used for the immunofluorescent staining of single-cell suspensions prepared from either lymphoid tissues, bone marrow, peripheral blood, or cultured cells. PharmingenStain Buffer (BSA) is useful for the dilution and application of fluorescent reagents as well as for the suspension, washing, and storage of cells destined for flow cytometric analysis (or fluorescence microscopy). Based on previous descriptions of staining media, PharmingenStain Buffer (BSA) was formulated as a neutral pH (pH 7.4)-buffered salt solution (i.e., DPBS) that is supplemented with 0.2% (w/v) bovine serum albumin (BSA) proteins. As such, PharmingenStain Buffer (BSA) is designed to maintain cell viability and maximize fluorescence signal intensities generated by pH-sensitive fluorochromes, e.g., fluoresceine isothiocyanate (FITC) . PharmingenStain Buffer (BSA) is useful for staining cells with biotinylated antibodies and fluorochrome-conjugated avidins because this staining medium contains no biotin . When present in staining media, free biotin can interfere with the binding of fluorescent-avidins to biotinylated antibodies that have complexed with their cognate, cell-associated antigens. Moreover, free biotin can bind to cells and contribute to an increase in the non-specific, background staining of cells that are exposed to fluorochrome-conjugated avidins. PharmingenStain Buffer (BSA) contains the metabolic inhibitor, sodium azide (NaN3). NaN3 inhibits the potential redistribution of cell surface antigens (e.g., due to shedding or internalization) caused by antibody crosslinking. NaN3 (in combination with maintenance of cold ambient temperatures) thereby prevents the potential loss of fluorescent signal intensities generated by immunofluorescently-stained cells during subsequent flow cytometric analysis(or fluorescence microscopy).

注意事项：

1.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.