MiCK-1 Cytokine Positive Control Cells

• 产品编号：BD-554652      品牌：BD-Pharmingen       原厂货号：554652
• 产品分类：细胞,细胞培养与转染试剂 > 细胞 > 哺乳动物细胞系
• 应用分类：

描述：

This suspension contains Mouse intracellular CytoKine-1 (MiCK-1) Positive Control Cells. The MiCK-1 cell suspension contains ~5 x 10^6
fixed, non-permeabilized mouse lymphoid cells. The suspension includes cells that express easily detectable levels of intracellular IL-2, TNF
(also known as TNF-α), and IFN-γ as determined by immunofluorescent intracellular cytokine staining and flow cytometry. MiCK-1 cell
suspensions were prepared by stimulating mouse spleen cells in the presence of a protein transport inhibitor. After stimulation, the cells were
harvested and were incubated with Fc Block™ [rat IgG2b,κ anti-mouse CD16/CD32 (FcγII/III receptor) antibody; Cat. No. 553142] to reduce
Fc receptor-mediated background staining. The cells were fixed and then stored in 10% dimethylsulfoxide and 90% fetal bovine serum at
-80°C. Each lot of MiCK-1 contains a measurable proportion of cytokine-producing cells. Representative flow cytometric results are shown
for typical staining of MiCK-1 cells. Data from individual lots of MiCK-1 cells may vary. Investigators should anticipate similar (though not
identical) results to those shown due to differences in staining methodology and in flow cytometers/cytometer settings.

原厂资料：

注意事项：

Store product at -80°C prior to use or for long term storage of stock solutions.
Rapidly thaw and quick-spin product prior to use.
Avoid multiple freeze-thaws of product.
This preparation contains no preservatives, thus it should be handled under aseptic conditions.