Interleukin-12 (IL-12) is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. The IL-12 p40 monomer shares amino acid sequence homology with the IL-6 receptor. It has been reported that activated PBMC produce a many fold excess of IL-12 p40 monomer over the bioactive p70 heterodimer. The IL-12 p40 monomer has been reported to inhibit binding of IL-12 p70 to the IL-12 receptor, but with 20X less effectiveness than the IL-12 p70 homodimer. Recombinant human IL-12 p40 protein was purified by immunoaffinity chromatography. The recombinant is > 95% pure, as detemined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 0.1 ng/µg of human IL-12 p40, as measued in a chromogenic LAL assay. This recombinant protein is routinely tested by ELISA. Other applications were tested at BD Biosciences Pharmingen during product development only or reported in the literature. Preparation and Storage
Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. Recombinant human IL-12 p40 monomer is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution (pH 7.4) and containing at least 1.0 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80˚C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 – 1.0 mg/ml carrier protein** such as human or bovine albumin, aliquoted and stored at -80˚C. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.
原厂资料：
Description
Interleukin-12 (IL-12) is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. The IL-12 p40 monomer shares amino acid sequence homology with the IL-6 receptor. It has been reported that activated PBMC produce a many fold excess of IL-12 p40 monomer over the bioactive p70 heterodimer. The IL-12 p40 monomer has been reported to inhibit binding of IL-12 p70 to the IL-12 receptor, but with 20X less effectiveness than the IL-12 p70 homodimer. Recombinant human IL-12 p40 protein was purified by immunoaffinity chromatography. The recombinant is > 95% pure, as detemined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 0.1 ng/µg of human IL-12 p40, as measued in a chromogenic LAL assay. This recombinant protein is routinely tested by ELISA. Other applications were tested at BD Biosciences Pharmingen during product development only or reported in the literature. Preparation and Storage
Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. Recombinant human IL-12 p40 monomer is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution (pH 7.4) and containing at least 1.0 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80˚C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 – 1.0 mg/ml carrier protein** such as human or bovine albumin, aliquoted and stored at -80˚C. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.
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