Hu IL-10 Recom 5ug

产品编号：BD-554611 品牌：BD-Pharmingen 原厂货号：554611
产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IL-10 Family 白细胞介素-10超家族
应用分类：细胞培养产品与服务 > 哺乳动物细胞培养 > 生长因子和纯化蛋白质 > 物种 > 人
　　　　　细胞培养产品与服务 > 哺乳动物细胞培养 > 细胞因子 > 白细胞介素

包装：

5ug

运保温度:

stored at -80°C

到货周期：

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描述：

Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 18.5 kD protein that shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which may be interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production. The net effect of IL-10 action appears to be the inhibition of proinflammatory T cell mediated immunity.

Formulation and Purity

Recombinant human IL-10 is supplied as a frozen, sterile-filtered aqueous buffered solution containing glycerol and biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant human IL-10 was purified by immunoaffinity chromatography and was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 0.1 ng per µg of hIL-10, as measured in a chromogenic LAL assay.

Preparation and Storage

Store product at -80°C prior to use or for long term storage of stock solutions.

Rapidly thaw and quick-spin product prior to use.Avoid multiple freeze-thaws of product.Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity. For in vitro biological assay use, we recommend carrier-protein concentrations of ≥ 1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should not be diluted to less than 50 μg/ml for long term storage. Note: Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

注意事项：

Store product at -80°C prior to use or for long term storage of stock solutions.
Rapidly thaw and quick-spin product prior to use.
Avoid multiple freeze-thaws of product.
Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use.
Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as
human or bovine albumin, aliquoted and stored at -80°C. Carrier proteins should be pre-screened for possible effects in an
appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or
possible blocking activity. For in vitro biological assay use, we recommend carrier-protein concentrations of ≥ 1 mg/ml. For use
as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at
indicated temperatures may result in a loss of activity. The product should not be diluted to less than 50 μg/ml for long term
storage. Note: Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier
proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.