Hu IL-1Bta Recom 5ug

产品编号：BD-554602 品牌：BD-Pharmingen 原厂货号：554602
产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IL-1 Family 白细胞介素-1超家族
应用分类：细胞培养产品与服务 > 哺乳动物细胞培养 > 生长因子和纯化蛋白质 > 物种 > 人
　　　　　细胞培养产品与服务 > 哺乳动物细胞培养 > 细胞因子 > 白细胞介素

包装：

5ug

运保温度:

Store product at -80°C prior to use or for long te

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描述：

Interleukin-1β (IL-1β) is a potent immunomodulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. This is the mature form of human IL-1β, a 17 kD protein containing 153 amino acid residues, representing amino acids A117 through S269 of the IL-1β precursor protein, expressed in E. coli.

Formulation and Purity

Recombinant human IL-1β is lyophilized from a solution comprised of 0.22 μm sterile-filtered aqueous buffered solution with no preservatives. Each vial contains 250 μg of bovine serum albumin, for a final ratio of carrier protein to human IL-1β of 50:1. The recombinant human IL-1β is ≥ 95% pure as determined by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 1.0 EU per µg of human IL-1β as determined by the LAL method.

Preparation and Storage

Store product at -80°C prior to use or for long term storage of stock solutions.

This preparation contains no preservatives, thus it should be handled under aseptic conditions.

The product can be reconstituted in sterile neutral buffer containing 0.5 - 10 mg/ml carrier protein such as human or bovine serum albumin, aliquoted and stored at –80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may affect experimental results due to toxicity, high endotoxin levels or possible blocking activity

注意事项：

Store product at -80°C prior to use or for long term storage of stock solutions.
This preparation contains no preservatives, thus it should be handled under aseptic conditions.
The product can be reconstituted in sterile neutral buffer containing 0.5 - 10 mg/ml carrier protein such as human or bovine
serum albumin, aliquoted and stored at –80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of
0.5 - 1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier
protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible
effects in an appropriate experimental system. Carrier proteins may affect experimental results due to toxicity, high endotoxin
levels or possible blocking activity.